Journal: Arthritis Research & Therapy

Article Title: Proposition of a novel animal model of systemic sclerosis induced by type V collagen in C57BL/6 mice that reproduces fibrosis, vasculopathy and autoimmunity

doi: 10.1186/s13075-019-2052-2

Figure Lengend Snippet: Changes in collagen fibers in the lung and skin lesions and immunohistochemical analysis of vascular injury in the lung following the Col V immunization in C57BL/6 mice. Immunofluorescence was performed to characterize collagen I (Col I), collagen III (Col III), and collagen V (Col V) in the lung ( a ) and skin ( b ). Mice receiving Col V exhibited increased deposition of Col I and Col V fibers in the lung and skin compared with control. The Picrosirius staining in skin samples showed total collagen in Col V-immunized mice and control ( b ). Immunohistochemical analysis was performed on the lung C57BL/6 mice receiving the treatment of Col V. Positive cells staining with endothelial markers VEGF and endothelin-1 and apoptosis marker caspase-3 were indicated by brown color ( c )

Article Snippet: The specimens were incubated overnight at 4 °C with rabbit polyclonal anti-human Col I (1:700, Rockland), anti-human Col III (1:200, Rockland), and anti-human Col V (1,1.000, Rockland) antibodies diluted in PBS and stained with an ALEXA FLUOR 488 goat anti-rabbit IgG antibody (Invitrogen) diluted 1:200 in a PBS solution containing 0.006% Evans blue.

Techniques: Immunohistochemical staining, Immunofluorescence, Staining, Marker

Journal: Arthritis Research & Therapy

Article Title: Proposition of a novel animal model of systemic sclerosis induced by type V collagen in C57BL/6 mice that reproduces fibrosis, vasculopathy and autoimmunity

doi: 10.1186/s13075-019-2052-2

Figure Lengend Snippet: Increased collagen fibers density in the lung and skin and increase expression of endothelial markers of vascular injury in mice following the immunization with Col V. Quantification of collagen I (Col I), collagen III (Col III), and collagen V (Col V) and endothelial markers in the lung ( a ) and Col I, Col III, and Col V in the skin ( b ) by Image Pro-Plus of mice immunized with Col V. The quantification of the Picrosirius staining showed collagen fine fibers increase in Col V-immunized mice compared with control. Data are mean ± s.e. ( n = 38) P < 0.05

Article Snippet: The specimens were incubated overnight at 4 °C with rabbit polyclonal anti-human Col I (1:700, Rockland), anti-human Col III (1:200, Rockland), and anti-human Col V (1,1.000, Rockland) antibodies diluted in PBS and stained with an ALEXA FLUOR 488 goat anti-rabbit IgG antibody (Invitrogen) diluted 1:200 in a PBS solution containing 0.006% Evans blue.

Techniques: Expressing, Staining

Journal: Arthritis Research & Therapy

Article Title: Proposition of a novel animal model of systemic sclerosis induced by type V collagen in C57BL/6 mice that reproduces fibrosis, vasculopathy and autoimmunity

doi: 10.1186/s13075-019-2052-2

Figure Lengend Snippet: Quantitative qRT-PCR analysis of collagen I (COL1A1 and COL1A2), collagen III (COL3A1) and collagen V (COL5A1 and COL5A2) genes expression in the lung ( a ) and skin ( c ) tissues, and endothelial expression of VEGF, Endothelin-1 and Caspase-3 in lung tissue ( b ) from C57BL/6 mice immunized with Col V. Data represent mean ± s.e ( n = 38) P < 0.05

Article Snippet: The specimens were incubated overnight at 4 °C with rabbit polyclonal anti-human Col I (1:700, Rockland), anti-human Col III (1:200, Rockland), and anti-human Col V (1,1.000, Rockland) antibodies diluted in PBS and stained with an ALEXA FLUOR 488 goat anti-rabbit IgG antibody (Invitrogen) diluted 1:200 in a PBS solution containing 0.006% Evans blue.

Techniques: Quantitative RT-PCR, Expressing

Journal: Arthritis Research & Therapy

Article Title: Proposition of a novel animal model of systemic sclerosis induced by type V collagen in C57BL/6 mice that reproduces fibrosis, vasculopathy and autoimmunity

doi: 10.1186/s13075-019-2052-2

Figure Lengend Snippet: ELISA assays showed elevated anti-collagen III, IV, and V antibodies ( a ) and immunofluorescence assays showed a higher levels of antinuclear antibodies (ANA) ( b ) in the serum from the mice immunized with Col V. The immunofluorescence shows ANA with a fine dotted and/or Golgi cytoplasmic pattern in serum from C57BL/6 mice immunized with Col V ( b , arrow). Data represent the mean ± s.e. ( n = 38). P < 0.05

Article Snippet: The specimens were incubated overnight at 4 °C with rabbit polyclonal anti-human Col I (1:700, Rockland), anti-human Col III (1:200, Rockland), and anti-human Col V (1,1.000, Rockland) antibodies diluted in PBS and stained with an ALEXA FLUOR 488 goat anti-rabbit IgG antibody (Invitrogen) diluted 1:200 in a PBS solution containing 0.006% Evans blue.

Techniques: Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Collagen-modified factors detection ( a ) Quantitative real-time PCR analysis of Col-I and Col-III mRNA expression of hPDLCs. n = 3 . ( b ) Western blot analysis of Col-I and Col-III protein levels of hPDLCs. n = 3 . Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. the control group, #P < 0.05 vs. the tensile stress group and &P < 0.05 vs. the laser group by one-way ANOVA

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Modification, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control